Publications
CONTRIBUTIONS TO SCIENTIFIC LITERATURE AND PROCEEDINGS
REFEREED JOURNAL PUBLICATIONS
Use of population genetics to derive non-recombinant Saccharomyces cerevisiae that grow using xylose as a sole carbon source, PV ATTFIELD and P. J.L. Bell (2006), FEMS Yeast Research, 6, 862-868.
Involvement of oxidative stress response genes in redox homeostasis, the level of reactive oxygen species, and ageing in Saccharomyces cerevisiae, T Drakulic, M Temple, R Guido, S Jarolim, M Breitenbach, PV ATTFIELD, and IW Dawes (2005), FEMS Yeast Research, 5, 1215-1228.
Yeast differentiation using histone promoter sequences, PJ BELL (2004), Letters in Applied Microbiology, 38, 388-392.
Fluorophores from fungi, DA Veal , PJ BELL, H Brown, H-Y Choi and P Karuso (2003), Microbiology Australia, 24, 12-14.
Eppicocconone, a novel fluorescent compound from the fungus Epicoccum nigrum, PJ BELL and P Karuso (2003), Journal of the American Chemical Society, 125, 9304-9305.
A fluorescent natural product for ultra sensitive detection of proteins in 1-D and 2-D gel electrophoresis, JA Macintosh, HY Choi, SH Bae, DA Veal, PJ BELL, BC Ferrari, DD van Dyk, NM Verrills, YK Paik and P Karuso (2003), Proteomics, 3, 2273-2288.
The application of PCR for the isolation of a lipase gene from the genomic DNA of an antarctic microfungus, JR Bradner, PJ BELL, VS Te'o, and KM Nevalainen (2003), Current Genetics, 44, 224-230.
Application of a novel fluorescent dye to the detection of Giardia cysts, BC Ferrari, PV ATTFIELD, DA Veal and PJL Bell (2003), Journal of Microbiologial Methods, 52, 133-135.
A flow cytometric method for determination of yeast viability and biomass in a brewery, AR Boyd, TS Gunasekera, PV ATTFIELD, K Simic, SF Vincent and DA Veal (2003), FEMS Yeast Research, 3, 11-16.
Potential for broad applications of flow cytometry and fluorescence techniques in microbiological and somatic cell analyses of milk, TS Gunasekera, DA Veal and PV ATTFIELD (2003), International Journal of Food Microbiology, 85, 269-279.
Inducible gene expression by non-culturable bacteria in milk after pasteurization, TS Gunasekera, A Sorensen, PV ATTFIELD, S Sorensen and DA Veal (2002), Applied and Environmental Microbiology, 68, 1988-1993.
Prospecting for novel lipase genes using PCR, PJL BELL, A Sunna, MD Gibbs, NC Curach, H Nevalainen and PL Bergquist (2002), Microbiology, 148, 2283-2291.
Comparison of fermentative capacities of industrial baking and wild type yeasts of the species Saccharomyces cerevisiae in different sugar media, PJL Bell, VJ Higgins and PV ATTFIELD (2001), Letters in Applied Microbiology, 32, 224-229.
Heterogeneity of stress gene expression and stress resistance amongst individual cells of Saccharomyces cerevisiae, PV ATTFIELD, HY Choi, DA Veal and PJL Bell (2001), Molecular Microbiology, 40, 1000-1008.
Generation of a novel Saccharomyces cerevisiae strain that exhibits strong maltose utilisation and hyperosmotic resistance using nonrecombinant techniques, VJ Higgins, PJ Bell, IW Dawes and PV ATTFIELD (2001), Applied and Environmental Microbiology, 67, 4346-4348.
A flow cytometry method for rapid detection and enumeration of total bacteria in milk, T Gunasekera, PV ATTFIELD and DA Veal (2000), Applied and Environmental Microbiology, 66, 1228-1232.
Flow cytometry for monitoring microbial cells, DA Veal, D Deere, B Ferrari, J Piper and PV ATTFIELD (2000), Journal of Immunological Methods, 243, 191-210.
Use of flow cytometry to monitor cell damage and predict fermentation activity of dried yeasts, PV ATTFIELD, S Kletsas, DA Veal, R van Rooijen and PJL Bell (2000), Journal of Applied Microbiology, 89, 207-214.
Evaluation of light scattering and autofluorescent properties of brewer's worts for flow cytometric analysis of yeast viability, A Boyd, PV ATTFIELD, DA Veal and SVincent (2000), Journal of Institute of Brewing, 106, 319-324.
Hyperosmotic stress response by strains of baker's yeasts in high sugar concentration medium, PV ATTFIELD and S Kletsas (2000), Letters in Applied Microbiology, 31, 323-327.
Intracellular concentration of exogenous glycerol by Saccharomyces cerevisiae provides for improved leavening of frozen doughs, DK Myers and PV ATTFIELD (1999), Food Microbiology, 16, 45-51.
Enhancement of maltose utilisation by Saccharomyces cerevisiae in medium containing fermentable hexoses, BW Hazell and PV ATTFIELD (1999), Journal of Industrial Microbiology and Biotechnology, 22, 627-632.
Genetic evidence that high uninduced maltase and maltose permease activities, governed by MALX3 transcriptional regulators, determines efficiency of gas production by baker's yeast in unsugared doughs, V Higgins, M Braidwood, P Bell, PH Bissinger, IW Dawes and PV ATTFIELD (1999), Applied and Environmental Microbiology, 65, 680-685.
Leu343Phe substitution in the MALX3 protein of Saccharomyces cerevisiae increases constitutivity and glucose insensitivity of MAL gene expression, V Higgins, M Braidwood, PH Bissinger, IW Dawes and PV ATTFIELD (1999), Current Genetics, 35, 491-498.
Facilitating functional analysis of the Saccharomyces cerevisiae genome using an EGFP based promoter library and flow cytometry, PJL Bell, IW Dawes and PV ATTFIELD (1999), Yeast, 15, 1747-1759.
Applications of flow cytometry to microbiology of food and beverage industries, P ATTFIELD, T Gunasekera, A Boyd, D Deere and D Veal (1999), Australasian Biotechnology, 9, 159-166.
Rapid cloning of theromalkalaophilic lipases from Bacillus spp., PJL BELL, H Nevalainen, HW Morgan and PL Bergquist (1999), Biotechnology Letters, 21,1003-1006.
Comparison of melibiose utilising baker's yeast strains produced by genetic engineering and classical breeding. SF Vincent, PJL BELL, P Bissinger and KMH Nevalainen (1999), Letters in Applied Microbiology, 28,148-152.
Loading of Saccharomyces cerevisiae with glycerol leads to enhanced fermentation in sweet bread doughs, DK Myers, VM Joseph, S Pehm, M Galvagno and PV ATTFIELD (1998), Food Microbiology, 15, 51-58.
A flow cytometric method for rapid selection of novel industrial yeast hybrids, PJL Bell, D Deere, J Shen, B Chapman, PH Bissinger, PV ATTFIELD and DA Veal (1998), Applied and Environmental Microbiology, 64, 1669-1672.
Stress co-tolerance and trehalose content in baking strains of Saccharomyces cerevisiae, JG Lewis, RP Learmonth, PV ATTFIELD and K Watson (1997), Journal of Industrial Microbiology and Biotechnology, 18, 30-36.
Influence of invertase activity and glycerol synthesis and retention on fermentation of media with a high sugar concentration by Saccharomyces cerevisiae, DK Myers, DTM Lawlor and PV ATTFIELD (1997), Applied and Environmental Microbiology, 63, 145-150.
The importance of stress tolerance to baker's yeast, PV ATTFIELD, BW Hazell and DK Myers (1997), Australasian Biotechnology, 7, 149-154.
Freeze/thaw stress response of the yeast Saccharomyces cerevisiae is growth-phase specific and controlled by nutritional status via the RAS-cAMP signal transduction pathway, J-I Park, C Grant, PV ATTFIELD and IW Dawes (1997), Applied and Environmental Microbiology, 63, 3818-3824.
Involvement of CIF1 (GGS1/TPS1) in osmotic response of Saccharomyces cerevisiae, BW Hazell, S Kletsas, H Nevalainen and PV ATTFIELD (1997), FEBS Letters, 414, 353-358.
Stress tolerance: the key to effective industrial baker's yeast, PV ATTFIELD (1997), Nature Biotechnology, 15, 1351-1357.
Tandemly Repeated 147 bp elements cause structural and functional variation in divergent MAL promoters of Saccharomyces cerevisiae, PJL BELL, VJ Higgins, IW Dawes and P H Bissinger (1997), Yeast, 13,1135-1144.
Flow cytometry and cell sorting for bakers yeast viability assessment and viable cell selection, D Deere, J Shen, G Vesey, P BELL, PH Bissinger, and D Veal (1997), Yeast, 14,147-160.
Proven efficacy and economics of a pollen substitute as a honeybee nutrient, P Ramm & PV ATTFIELD (1996), Australasian Beekeeper, 97, 408-416.
Evidence that the Saccharomyces cerevisiae CIF1 (GGS1/TPS1) gene modulates heat shock response positively, B Hazell, H Nevalainen and PV ATTFIELD (1995), FEBS Letters, 377, 457-460.
A two-reporter gene system for the analysis of bi-directional transcription from the divergent MAL6T-MAL6S promoter in Saccharomyces cerevisae, PJL BELL, PH Bissinger, RJ Evans and IW Dawes (1995), Current Genetics, 28, 441-446.
Concomitant appearance of thermotolerance and storage of trehalose in Saccharomyces cerevisiae during early respiratory phase of batch-culture is CIF1-dependent, PV ATTFIELD, S Kletsas and B Hazell (1994), Microbiology, 140, 2625-2632.
The need for consistent nomenclature and assessment of growth phases in diauxic cultures of Saccharomyces cerevisiae, JG Lewis, CJ Northcott, RP Learmonth, PV ATTFIELD and K Watson (1993), Journal of General Microbiology, 139, 835-839.
Construction of Saccharomyces cerevisiae strains that accumulate relatively low concentrations of trehalose, and their application in testing the contribution of the disaccharide to stress tolerance, PV ATTFIELD, A Raman and CJ Northcott (1992), FEMS Microbiology Letters, 94, 271-276.
Trehalose accumulates in Saccharomyces cerevisiae during exposure to agents that induce heat shock response, PV ATTFIELD (1987), FEBS Letters, 225, 259-263.
Analysis of the ruv locus of Escherichia coli K12 and identification of the gene product, PV ATTFIELD, FE Benson and RG Lloyd (1985), Journal of Bacteriology, 164, 276-281.
Involvement of Escherichia coli K12 recombination functions in elimination of multicopy plasmid R6K by DNA damaging agents, PV ATTFIELD and RJ Pinney (1985), FEMS Microbiology Letters, 28, 287- 291.
Elimination of multicopy plasmid R6K by bleomycin, PV ATTFIELD and RJ Pinney (1985), Antimicrobial Agents and Chemotherapy, 27, 985-988.
Repair of DNA double strand breaks in Escherichia coli K12 requires a functional recN gene, SM Picksley, PV ATTFIELD and RG Lloyd (1984), Molecular and General Genetics, 195, 267-274.
Genetic analysis and molecular cloning of the Escherichia coli ruv gene, CE Shurvinton, RG Lloyd, FE Benson and PV ATTFIELD (1984), Molecular and General Genetics, 194, 322-329.
Comparison of the Escherichia coli umu+ encoded function with R46 mediated error prone repair in DNA damaged cells, PV ATTFIELD and RJ Pinney (1984), Mutation Research, 139, 101-105.
Plasmid R46 fails to protect Escherichia coli against double strand DNA binding agents but increases their mutagenic activities, PV ATTFIELD and RJ Pinney (1983), Mutation Research, 107, 1-12.
Plasmid mediated protection against bleomycin is polA+ dependent, PV ATTFIELD and RJ Pinney (1982), Journal of General Microbiology, 128, 539-547.
BOOK CHAPTERS
Genetics and classical genetic manipulation of industrial yeasts, PV ATTFIELD and PJL Bell (2003), in, Functional Genetics of Industrial Yeasts, (JH de Winde, ed), Topics in Current Genetics, (S Hohmann, Series Ed), 2, pp17-55. Springer-Verlag, Heidlelberg and New York.
Genetic improvement of baker's yeasts, PV ATTFIELD and PJL Bell (2003), in, Applied Mycology and Biotechnology, (DK Arora and GG Khachatourians, eds.), Vol3: Fungal Genomics. Chapter 10, pp 213-240. Elsevier, Amsterdam.
Comparison of the methylene blue assay with a new flow-cytometric method for determining yeast viability in a brewery, A Boyd, T Gunasekera, P ATTFIELD, K Simic, S Vincent and D Veal (2003), in, Brewing Yeast Fermentation Performance, (K Smart ed.), 2nd edition, pp 174-179. Blackwell Science, Oxford, UK.
Physiological and molecular aspects of hyperosmotic stress tolerance in yeasts, PV ATTFIELD (1998), in, Recent Research Developments in Microbiology, (SG Pandalai, Managing ed.), Vol. 2-1998 Part II, pp. 427-441. Research Signpost, Trivandrum, India.
Genetic engineering of yeasts: principles and applications, RJ Evans and PV ATTFIELD (1989), in, Biotechnology and the Food Industry, (PL Rogers and GH Fleet, eds.), Chapter 3, pp. 33-60. Gordon & Breach Science. Publications, NY.
COMMUNICATIONS TO NATIONAL AND INTERNATIONAL CONFERENCES, SYMPOSIA AND MEETINGS
Bioconversion of lignocellulosic biomass to fuel and food, Bioenergy Australia 2009 - From Opportunity to Implementation, Gold Coast, Queensland, December 2009, Paul Attfield.
Novel yeasts to enable a cellulosic food and fuel biorefinery, Yeast: Products and Discovery 09, Adelaide, South Australia, December 2009, Philip Bell.
Microbiogen's Food and Fuel Biorefinery, Ausbiotech 2009, Melbourne, Victoria, Australia 2009, Geoffrey Bell.
Novel yeasts to enable a cellulosic ethanol industry, Bioenergy Australia, Gold Coast, Queensland, Australia, November 2007, Philip Bell.
A different approach to developing yeasts for biomass conversion, Platts Cellulosic Ethanol and 2nd Generation Biofuels Conference, Chicago, USA, October 2007, Paul V Attfield.
Breakthrough in yeasts for making bio-ethanol from lignocellulosics, ISAF XV International Symposium on Alcohol Fuels, San Diego, USA, September 2005, P JL Bell and PV Attfield.
Potentiating classical yeast strain development, 11th International Congress on Yeasts, Rio de Janeiro, Brazil, August 2004, P Attfield.
Histone locus provides a target for rapid identification of Candida species, Australian Society for Microbiology & Australasian Mycological Society, Sydney, Australia, September 2004, G Pecenpetelovska, P Bell, K Marszewska, P Attfield and W Meyer.
Histone gene promoter region as a target for rapid identification of clinical yeast, 11th International Congress on Yeasts, Rio de Janeiro, Brazil, August 2004, PJL Bell, G Pecenpetelovska,, K Marszewska, PV Attfield and W Meyer.
Monitoring Heterogeneity: Improving Consistency of Industrial Yeasts, Yeast Products and Discovery Conference, Barossa Valley, South Australia, April 2004, P Attfield.
Metabolic activity in filamentous fungi can be analysed by flow cytometry, 22nd Fungal Genetics Conference, Asilomar Pacific Grove, California, USA, March 2003, JR Bradner, PV Attfield and KM Nevalainen.
The absence of oxidative stress genes leads to changes in cell redox state, XXth International Conference on Yeast Genetics and Molecular Biology, Prague, Czech Republic, August 2001, T Drakulic, VJ. Higgins, PV. Attfield and IW Dawes.
Comparison of the methylene blue assay with a new flow cytometric method for determining yeast viability in a brewery, 3rd Brewing Yeast Fermentation Performance Congress, Oxford, UK, September 2001, Andrew Boyd, Thusitha Gunasekera, Paul Attfield, Kristina Simic, Scott Vincent and Duncan Veal.
Is glycogen a useful indicator of brewer's yeast vitality? American Society for Microbiology 101st General Meeting, Orlando, Florida, USA, May 2001. A Boyd, T Gunasekera, P Attfield and D Veal.
Oxidative stress response genes are involved in maintenance of normal cell redox state, 22nd Lorne Annual Genome Conference, Lorne, Australia, February 2001. T Drakulic, VJ Higgins, PV Attfield and IW Dawes.
Use of recombinant DNA techniques to produce new and useful non-genetically modified yeasts, Yeast Products and Discovery Conference, South Stradbroke Island, Australia, June 2000. VJ Higgins, PV Attfield and IW Dawes.
Measuring Microbial Viability. How to do it and what does it mean? Australian Society for Microbiology, Cairns, Australia, July 2000. Duncan Veal, Paul Attfield, Matthias Dorsch, Michelle Power, Thusitha Gunasekera, Andrew Boyd, Gunnar Oregaard and Soren Sorensen.
In situ monitoring of stress response in individual yeast cells, 10th International Symposium on Yeasts, Arnhem, Holland, August - September 2000. PV Attfield, S Kletsas, H-Y Choi, DA Veal and PJL Bell.
Investigation of the cellular redox state in S. cerevisiae, Annual Meeting of the Society for Free Radical Research Australasia: Oxidants, Antioxidants and Nutrition, Wellington, New Zealand, December 2000. T Drakulic, N Alic, V Higgins, PV Attfield and IW Dawes.
Elucidation of stain efficacy for protein identification by PMF, Australian Electrophoresis Society 7th Annual Conference, Sydney, Australia, November 2000. PG Hains, DD van Dyk, NM Verrills, AM Connolly, PV Attfield, BJ Walsh.
Flow cytometry in the brewery, 26th Convention of the Institute of Brewing Asia Pacific Section, Singapore, March 2000. S Vincent, A Boyd, P Attfield, P Rogers and D Veal.
Baker's Yeast - a stressful business, Yeast Products and Discovery Conference, South Stradbroke Island, Australia, June 2000. P Attfield and P Bell.
Using flow cytometry for classical and molecular genetics of yeasts, Yeast Products and Discovery Conference, South Stradbroke Island, Australia, June 2000. P Bell and P Attfield.
Flow cytometry and fluorescence for studying physiology and gene expression in yeast, Yeast Products and Discovery Conference, South Stradbroke Island, Australia, June 2000. PV Attfield, H-Y Choi, S Kletsas, T Gunasekera, A Boyd, PJL Bell and DA Veal.
Making yeasts more tolerant to stress. Combined meeting of the Australian Institute of Food Science and Technology and The Australian Society for Microbiology, Sydney, Australia, February 1999. P Attfield.
New approach to milk microbiology. 14th Annual Conference of the Dairy Industry Association of Australia, Sydney, Australia, May 1999. P Attfield, T Gunasekera and DA Veal.
Application of flow cytometry to analyses of yeast vitality and metabolism. Carlton and United Breweries Symposium on Brewing Yeast, Melbourne, Australia, June 1999. P Attfield and A Boyd.
Amino acid changes in the yeast maltose regulatory protein (MalX3p) relieve glucose repression of maltose expression. 20th Annual Conference on the Organisation and Expression of the Genome, Lorne, Australia, February 1998. V Higgins, P Bell, P Attfield and I Dawes.
Freeze/thaw stress response of the yeast Saccharomyces cerevisiae is growth-phase specific and controlled by nutritional status via the RAS-cAMP signal transduction pathway. 19th Annual Conference on the Organisation and Expression of the Genome, Lorne, Australia, February 1997. J-I Park, C Grant, P Attfield and I Dawes.
Development of flow cytometric methods for understanding stress tolerance in baker's yeast. 20th Annual Australasian Flow Cytometry Group Meeting, Auckland, New Zealand, October 1997. B Chapman, D Deere, P Bell, J Shen, D Veal and P Attfield.
A flow cytometric method for rapid selection of novel industrial yeast hybrids. 20th Annual Australasian Flow Cytometry Group Meeting, Auckland, New Zealand, October 1997. J Shen, D Deere, P Bell, B Chapman, P Attfield, P Bissinger and D Veal.
Development of a vector suitable for the analysis of divergent promoters, Lorne Genome conference, Lorne, Australia, February 1997. PJL Bell, V Higgins, J Shen and IW Dawes.
Functional analysis of a novel MalX3p transcriptional regulator, Lorne Genome conference, Lorne, Australia, February 1997. VJ Higgins, PJL Bell, MAT Braidwood, PH Bissinger, and IW Dawes.
Is lipid composition of Saccharomyces cerevisiae affected by cif1 mutation? 9th International Symposium on Yeasts, Sydney, Australia, August 1996. C Northcott and P Attfield.
Does CIF1 (GGS1/TPS1) modulate hyperosmotic stress response in Saccharomyces cerevisiae? 9th International Symposium on Yeasts, Sydney, Australia, August 1996. B Hazell, H Nevalainen and P Attfield.
Yeast adaptation to hyperosmotic, high fermentable sugar media. 9th International Symposium on Yeasts, Sydney, Australia, August 1996. D Myers, D Lawlor and P Attfield.
Flow cytometry as a tool for selecting for live yeast cells from populations of mixed viability, 9th International Symposium on Yeasts, Sydney, Australia, August 1996. D Deere, J Shen, PJL. Bell, Y Li, PH Bissinger, and D Veal.
Divergent expression from the MAL genes of S. cerevisiae is controlled by 147 bp attenuator elements, 9th International Symposium on Yeasts, Sydney, Australia, August 1996. PJL Bell, IW Dawes and. PH Bissinger.
Trehalose accumulation and membrane fluidity changes during heat shock in yeasts. Australian Society for Biochemistry & Molecular Biology and Australian Society of Plant Physiologists, Canberra, Australia, September 1996. R Learmonth, S Carlin and P Attfield.
Divergent expression from polymorphic MALT/MALS promoters isolated from a single strain of bakers' yeast, Lorne Genome conference 1995. PJL Bell, VJ Higgins, IW Dawes and PH Bissinger.
Growth phase-dependent role of trehalose in intrinsic and induced thermotolerance of Saccharomyces cerevisiae, Australian Society for Biochemistry & Molecular Biology, Gold Coast, Australia, September 1994. B Hazell, S Kletsas, H Nevalainen and P Attfield.
Trehalose: Is it a stress protectant? 2nd Congress of the Asian-Pacific Organisation for Cell Biology, Sydney, Australia, October 1994. P Attfield and S Kletsas.
Stress tolerance in yeast: Relationships between five stresses and growth state, Australian Society for Biochemistry & Molecular Biology, Canberra, Australia, September 1991. J Lewis, C Northcott, R Learmonth, P Attfield and K Watson.
Genetic engineering of yeast, University of New South Wales Symposium on, Biotechnology: Opportunities for the Australian Food Industry, Sydney, Australia, February 1987. R Evans and P Attfield.
Genetic analysis and regulation of inducible recombination in Escherichia coli, Cold Spring Harbor Symposium on Quantitative Biology, Cold Spring Harbor, USA, August 1984. R Lloyd, S Picksley, F Benson and P Attfield.
Inhibition of plasmid R6K DNA synthesis by bleomycin in vivo and in vitro, British Pharmaceutical Society Conference, London, UK, September 1983. P Attfield and RJ Pinney.
Elimination of plasmid R6K by DNA damaging agents, British Pharmaceutical Society Conference, London, UK, September 1983 P Attfield and R Pinney.
Host umuC gene function mimics R46 mediated protection of Escherichia coli exposed to DNA damage, British Pharmaceutical Society Conference, Edinburgh, UK, September 1982. P Attfield and RJ Pinney.
Plasmid R46 protects E.coli against single strand but not double strand DNA damage, British Pharmaceutical Society Conference, Brighton, UK, September 1981. P Attfield and RJ Pinney.
R Plasmids mediate protection and sensitivity to bleomycin, British Pharmaceutical Society Conference, Newcastle, UK, September 1980. P Attfield and RJ Pinney.